Opioids made using yeast instead of poppies
Process needs to be more efficient to be commercially viable
Scientists have invented a speedy method to make potent painkilling opioids using bioengineered baker's yeast instead of poppies, but need to fine-tune the process to make it commercially viable, according to a study published on Thursday.
The new method, if it can be made more efficient, could significantly change the multibillion-dollar pain medication manufacturing business, but raises concerns about aggravating the growing problem of opioid abuse.
This same type of approach potentially also could be used to make other currently plant-based medicines for fighting cancer, infectious diseases and chronic illnesses.
The scientists said they altered the yeast's genetic make-up in a way that coaxed the cells to convert sugar into two opioids - hydrocodone and thebaine - in three to five days.
Hydrocodone, which shuts down pain receptors in the brain, and related chemicals like morphine and oxycodone are part of a group of painkilling drugs called opioids produced from the opium poppy. Making opioids from opium poppies can take more than a year.
"This is important because, with further development, it may provide an alternative supply for these essential medicines and allow greater access for most of the global population that currently has insufficient access to pain medication," said Stanford University bioengineering professor Christina Smolke, who led the research published in the journal Science.
The researchers said they recognized their findings on a quicker process to make opioids could generate concern about worsening the growing abuse of these painkillers.
Smolke's team inserted DNA into the yeast that encodes instructions for the cells to make the enzymes necessary to perform the sugar conversion steps.
The baker's yeast was engineered with 23 genes from six different organisms: the brown rat, the goldthread herb, the California poppy, the Iranian poppy, the opium poppy and a soil bacterium called Pseudomonas putida.
The process unveiled by Smolke's team represents "proof of principle experiments" showing that the bioengineered yeast can produce small amounts of opioid painkillers from scratch, Smolke said. Currently, about 4,400 gallons of bioengineered yeast would be needed to yield a single dose of the medication.
Smolke said the process needs to be improved to make it efficient enough for commercial production, saying this could take several years.
Stanford University holds the patents on the technology described by the researchers, who have formed a company to pursue the commercial applications.
This past May, researchers from the University of California, Berkeley and Concordia University in Montreal announced they had figured out how to do the last remaining step needed to produce opioids like morphine from yeast, making it theoretically possible.