Navratilova, 47, will compete as a wild-card entry in her first Grand Slam singles action since retiring from singles play in 1994.
The tournament commences May 24 at Roland Garros Stadium in Paris.
Navratilova captured the French Open in 1982 and 1984 for two of her 18 Grand Slam titles.
Martina Navratilova is entering the singles draw at the French Open.(AP Photo)
She also won nine Wimbledons, four U.S. Opens and three Australian Opens.
Navratilova's last singles match in a Grand Slam was a 6-4, 3-6, 6-3 loss to Conchita Martinez in the final of Wimbledon back in 1994.
She decided to stop playing singles later that year, following a 6-4, 6-2 loss to Gabriela Sabatini in the season-ending WTA Championships.
Navratilova retired the winningest singles player in WTA history, setting career records with 1,438 wins in 1,650 matches and $20,344,061 US in prize money.
Her 167 singles and nine Wimbledon titles are also records plus she reigned as the world's No. 1 tennis player in 1978-79 and again from 1982-1986.
Navratilova returned to singles tennis at Eastbourne in 2001 upon losing a bet with her trainer.
Despite a 7 1/2 year absence, she won 6-1, 4-6, 6-2 over Tatiana Panova, the world's 22nd-ranked player, to become the oldest (45 years, eight months) winner of a singles match on the WTA Tour.
Navratilova was born in Czechoslovakia but became an American citizen on July 21, 1981, after defecting six years earlier.
She was inducted into the International Tennis Hall of Fame in 2000.
with files from Sports Network
