Juventus took a giant step towards a third straight Serie A title after beating Fiorentina 1-0 on Sunday to extend its lead at the top of the standings to 14 points with second-placed Roma losing 1-0 at Napoli.
Roma has a game in hand but now second place is even at risk for the capital side as Napoli moved to within three points with the win.
In Turin. Kwadwo Asamoah scored a brilliant goal three minutes from halftime to give Juventus the lead.
"It wasn't an easy game and there's a lot of credit in having won it," Juventus coach Antonio Conte said. "We created a lot in the first half, In the second tiredness set in a bit, we had a lot of international players on the pitch who played during the week.
"The wins against Milan and today have put pressure on those behind us. I feel we're 50% champions, there are still 11 matches left. We shouldn't give up, like that those behind us will convince themselves they can't do it."
Unlike the first match this season where Juventus squandered a two-goal lead against Fiorentina to lose 4-2 — its only league defeat this campaign — the Bianconeri held on to record its 14th home league victory. It has a perfect record at the Juventus Stadium this season.
Inter Milan is only a point behind fourth-placed Fiorentina after beating Torino 1-0. Parma, which has a game in hand, is a point further back following its 2-0 victory over Hellas Verona.
Lazio lost 1-0 at home to Atalanta in a match which saw Antonio Candreva sent off for two bookings.
At the other end of the table, Livorno wasted a two-goal lead to lose a relegation battle 4-2 at Sampdoria.
Elsewhere, Chievo Verona secured a 2-1 win over Genoa, while Bologna and Sassuolo played out a goalless draw.
It was the first of three matches in less than two weeks between Juventus and Fiorentina as they meet in the Europa League.
"We came into the game more and more in the second half, the team became more confident and we had the chance to equalise, it's a pity," Fiorentina coach Vincenzo Montella said.
"I think we had a great second half. We have to pick up from there. On Thursday we have the chance to have our say and show what we can do."
Juventus broke the deadlock when Asamoah cut in from the left and carved out space between four Fiorentina defenders before unleashing a stunning effort which took a slight deflection before going in off the inside of the far post.
Modibo Diakite's effort was ruled out for a tight offside decision and Mario Gomez had a clear chance on the hour mark when he was left unmarked at the far post but the Germany striker, who is still not back to his best following five months out with injury, headed a cross wide.
Fiorentina was improving and went closest 11 minutes from time when substitute Ryder Matos headed off the crossbar shortly after coming off the bench.
Napoli had beaten Roma 3-0 the last time the two sides met at the Sao Paolo, in the Italian Cup semifinal.
Jose Maria Callejon scored in that match and he did so again eight minutes from time, heading Faouzi Ghoulam's cross in off the underside of the crossbar.
Inter is sneaking up on Fiorentina after picking up only its third win in 2014 and stretching its unbeaten run to five.
Inter dominated and took a deserved lead in bizarre circumstances as Rodrigo Palacio appeared to be trying to nod Esteban Cambiasso's cross back across the face of goal but it went in at the far post.
Parma and Verona were level on points at the start of the match and knew the victor would boost its hopes of qualifying for the Europa League.
Jonathan Biabiany got a decisive touch to deflect Massimo Gobbi's snap shot into the net off the inside of the right post in the 20th.
Parma sealed the result in stoppage time as Antonio Cassano stole the ball off Verona goalkeeper Rafael and, although his curling shot crashed off the post, Ezequiel Schelotto was on hand to follow up.
Livorno appeared to be heading for all three points after a double from Ibrahima Mbaye had given it a two-goal lead at halftime.
However, Sampdoria was level eight minutes after the break.
First, Francesco Bardi pulled off a good save to parry Roberto Soriano's effort but halftime substitute Nenad Krsticic bundled in the rebound and four minutes later Livorno defender Federico Ceccherini put the ball into his own net.
Stefano Okaka and Manolo Gabbiadini sealed the result.
Livorno remained in the relegation zone, two points behind Bologna and three below Chievo.