FIFA is not allowing Brazil and Portugal to train at the stadium in Durban to preserve the field for their final group stage match on Friday.
The Brazilian football federation says the teams will need to use other facilities in Durban to prepare for the match, which is likely to decide the Group G winner.
FIFA has been cancelling practices at some of the World Cup venues to avoid damaging the fields.
Brazil and Ivory Coast also could not train at Soccer City, and Germany and Serbia were kept from practising in Port Elizabeth.
Brazil leads Group G with six points, two more than Portugal. Ivory Coast has one point and North Korea zero.