Princess Irina Walker, daughter of the last king of Romania, has pleaded not guilty to operating a cockfighting business with her husband at their Oregon ranch.
She entered the plea at a hearing on Friday.
Government prosecutors warned that Walker is a flight risk because she was born in Switzerland and could be granted asylum in that country.
Judge John V. Acosta ordered her to surrender her passport and told her not to leave her home county, except to go to nearby Hermiston for groceries and medical appointments.
A total of 18 people have been charged in the case.
Walker's daughter, Angelica Kreuger, said her mother loves animals and would never watch a chicken die in a fight.