The intersection of Spadina Avenue and Dundas Street West will be shut down as of Monday, so that needed TTC and watermain construction work can be completed.
The intersection is expected to be closed until Aug. 11.
The temporary closure means that drivers will have to take other routes.
One major change to TTC service will involve the splitting of the 510 Spadina route into two halves while the work is being done over the next two weeks.
One half will run between Spadina station and Baldwin Street, while the other half will operate between Sullivan Street and Queen's Quay.
Passengers needing to go from Baldwin Street to Sullivan Street, or vice-versa, will have to carry a transfer and walk the distance of the gap between the two halves of the service.
Click on the video below to see a more in-depth explanation on the changes from the TTC's Andy Byford and Brad Ross.
Mobile users can view the video by clicking here.
Short-term pain, long-term gain
Grace Lau of the Toronto Chinatown Business Improvement Area said while the work will disrupt businesses during the peak tourist season, she believes it will benefit the area in the long run.
"We realized that this is probably the only time it can be done, so we have to bite the bullet," she said in an interview on CBC Radio's Metro Morning on Monday. "Short-term pain, long-term gain. That's what we're hoping for."
The work will clear the way for the TTC's new streetcars to operate on the 510 Spadina route. Lau said she expects the more modern, higher capacity streetcars will help bring more shoppers to Chinatown.