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Cyclists raise their bikes in unison during the memorial for Darcy Allan Sheppard. ((Victoria Foley/CBC))

Cyclists in Toronto staged another demonstration on a major downtown street Wednesday during afternoon rush hour, part of the aftermath of a fatal encounter between a bike courier and former Ontario attorney general Michael Bryant.

Riders took part in a memorial ride for Darcy Allan Sheppard around 5 p.m. ET along Bloor Street between Bay Street and Avenue Road. Dozens of riders occupied two eastbound lanes of the four-lane street. At times, they rang their bells and raised their bicycles over their heads in unison.

In a release, the Toronto Cyclists union said the case is "yet another painfully clear example of the need to better incorporate bikes/cyclists into our transportation network, to better educate all Torontonians about the fact that cyclists have a right to be on the roads, and that we have a responsibility to share this public space, and have more respect for our fellow citizens, cyclists, pedestrians and drivers alike."

The memorial ride came one day after about 100 bicycle riders staged a brief, impromptu protest near the site of the collision by laying down their bicycles on Bloor Street, blocking it off.

Sheppard's death on Monday night was the culmination of an escalating series of events that began after his bicycle was involved in what police called a "minor collision" at around 9:45 p.m. ET with Bryant's Saab convertible.

Witnesses said Sheppard got off his bicycle and hung on to Bryant's car as the former attorney general sped away.

Sheppard lost his grip and fell under the car. He was run over and badly injured. He died a few hours later at Toronto's St. Michael's Hospital.

Bryant, 43, has been charged with criminal negligence causing death and dangerous operation of a motor vehicle causing death.

Police Sgt. Tim Burrows said Bryant had not been drinking. After spending a night in jail, he was released under unspecified conditions on his own recognizance pending a court appearance Oct. 19.