Her office said Thursday she will not be running in this fall's municipal election.
- Ottawa's city election campaign officially begins
- Rainer Bloess, longtime Ottawa councillor, won't run again
- Osgoode councillor Doug Thompson to retire
Holmes' biography on her website said she was first elected to city council in 1982 after a career of volunteering and teaching physical education.
She was born in Montreal and went to McGill for a physical education degree, having also lived in Halifax.
Holmes had been listed as a nominated candidate on the city's website alongside five others.
Election day for local governments across Ontario is Oct. 27.