The Edmonton parents previously accused of starving their two-year-old twin daughters appeared briefly in court Friday morning to face new charges.
Each is now facing charges of second-degree murder and criminal negligence causing death.
The parents, who can't be named, wore leg shackles and standard prison clothing for the appearance.
As the two have been ordered not have contact with one another, a plexiglass barrier was placed in the prisoner's box between them.
The pair did not look at each other.
The girls — allegedly starved and neglected since birth — were taken from their south-side home last May, weighing 5.9 kilograms and 7.3 kilograms.
One of the girls, who can only be identified as "M," died in September 2012 after a judge ordered she be taken off life support.
A preliminary hearing is scheduled for June 17-28.