Inmates at the downtown remand centre began the move to the new facility in north Edmonton Friday.
Sheriff vehicles and correctional service vans began picking up prisoners at 3 p.m.
About 800 inmates are being transferred to the new Edmonton Remand Centre on 127th Street, north of the Anthony Henday.
While the old facility was over capacity, the new jail can hold almost 2,000 prisoners.
However the union representing correctional officers has raised concerns about the new centre.
The Alberta Union of Provincial Employees maintains that contract work is not finished and new guards may not be properly trained.
CBC sources suggested guards might take job action such as setting up pickets to prevent correctional services vehicles from leaving the old remand, but that did not happen.